在基因组时代，基因表达的分析已经成为许多实验室的一个关键要求。但一直没有全面的战略、概念和技术信息来源来指导这项往往复杂的任务。真核生物的转录调控》满足了这一需求。本书由加州大学洛杉矶分校医学院的Michael Carey和Stephen Smale两位经验丰富的研究人员撰写，部分内容基于冷泉港实验室教授的基因表达课程，直接解决实验室研究新分离基因的调控和新转录因子的生物化学的所有问题。 第1章回顾了RNA聚合酶II转录领域的最先进技术；第2-10章是调查新发现基因的调控机制的系统指南；第11-15章讨论了合成重组转录因子、分析其结合情况和破译其作用方式的方法。《真核生物的转录调控》不仅揭示了要做什么，而且揭示了为什么，以及如何正确计划以获得成功。这种方法是研究生、医生科学家、博士后和其他进入该领域的人的理想选择，但对在特定系统中进行深入研究的成熟调查人员也很有价值。 真核生物的转录调控》是一本强大的分子生物学高级教学教科书：用大量插图补充清晰的文字，将方法放在基础理论的背景下，对实验策略提出专家建议，鼓励研究设计中的创造性，逐步解释基本技术的协议，并对故障排除提出广泛建议。
In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor.Chapter 1 reviews the state-of-the-art in the RNA polymerase II transcription field;Chapters 2-10 are a systematic guide to the investigation of regulatory mechanisms for newly identified genes;Chapters 11-15 discuss approaches for synthesizing recombinant transcription factors, analyzing their binding, and deciphering their mode of action.Transcriptional Regulation in Eukaryotes reveals not only what to do but why, and how to plan properly for success. This approach is ideal for graduate students, physician-scientists, postdocs, and others entering the field, but is also valuable for established investigators undertaking in-depth studies in specific systems.Transcriptional Regulation in Eukaryotes is a powerful textbook for advanced instruction in molecular biology:supplementing clearly written text with extensive illustrationsputting methods in the context of underlying theorygiving expert recommendations on experimental strategiesencouraging creativity in investigative designexplaining protocols for essential techniques step by step, with extensive advice on troubleshootingThis important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.
